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KMID : 0545120180280030418
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 3 p.418 ~ p.424
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Biotransformation of Fructose to Allose by a One-Pot Reaction Using Flavonifractor plautii D-Allulose 3-Epimerase and Clostridium thermocellum Ribose 5-Phosphate Isomerase
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Lee Tae-Eui
Shin Kyung-Chul
Oh Deok-Kun
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Abstract
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D-Allose is a potential medical sugar because it has anticancer, antihypertensive, antiinflammatory, antioxidative, and immunosuppressant activities. Allose production from fructose as a cheap substrate was performed by a one-pot reaction using Flavonifractor plautii D-allulose 3-epimerase (FP-DAE) and Clostridium thermocellum ribose 5-phosphate isomerase (CT-RPI). The optimal reaction conditions for allose production were pH 7.5, 60°C, 0.1 g/l FPDAE, 12 g/l CT-RPI, and 600 g/l fructose in the presence of 1 mM Co2+. Under these optimized conditions, FP-DAE and CT-RPI produced 79 g/l allose for 2 h, with a conversion yield of 13%. This is the first biotransformation of fructose to allose by a two-enzyme system. The production of allose by a one-pot reaction using FP-DAE and CT-RPI was 1.3-fold higher than that by a two-step reaction using the two enzymes.
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KEYWORD
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Allose, allulose, Clostridium thermocellum ribose 5-phosphate isomerase, Flavonifractor plautii, D-allulose 3-epimerase
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